Rac GTP 活性抗体/ 抗体现货/高质量/ Rac-GTP 小鼠单抗/费斯德

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武汉市

武汉费斯德生物科技有限公司

普通会员

第 2 年

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产品详情（武汉市Rac-GTP 小鼠单抗/费斯德厂家）

分类：抗体

级别：其它

产品规格： 30 微升

关键词：武汉市Rac-GTP 小鼠单抗/费斯德厂家

货号: 26903

规格: 30 ?L

基因符号: RAC1

描述: Anti-Rac1-GTP Monoclonal Antibody

背景介绍: Small GTPases are a super-family of cellular signaling regulators. Rac belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene tranion. GTP binding increases the activity of Rac, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).

免疫原: Recombinant full length protein of active Rac1

经过测试的应用: IP, IHC, IF

推荐稀释比: IP: 1 ?g for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250

浓度: 1 mg/ml

宿主: Mouse

形式: Liquid

克隆性: Monoclonal

亚型: IgM

纯度: Purified from ascites

防腐剂: No

成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

种属反应性: Anti-Rac1-GTP monoclonal antibody recognizes active Rac1 from vertebrates.

储存条件: Store at -20°C. Avoid repeated freezing and thawing

免疫沉淀/蛋白质印迹:

Rac Activation Assay. MEF cells were treated with (lane 2) or without (lane 1) PDGF. Cell lysates were incubated with an anti-Rac-GTP monoclonal antibody (Cat. # 26903) (top panel). Th precipitated active Rac was immunoblotted with an anti-Rac rabbit polyclonal antibody (Cat. # 21003). The bottom panel shows the Western blot with anti-Rac of the cell lysates used (5% of that used in the top panel).

免疫荧光:

Immunofluorescence for the active Rac using Anti-Rac1-GTP Mouse Monoclonal Antibody [26903] shows Rac-GTP immunolabeling (green) in combination with cofilin (red) on brain tissue sections. The tissue sections were fixed with -20 ?C methanol or 4% paraformaldehyde (fixation time 1 hr) and stained with antibody at 1: 1000 in 0.1M Phosphate buffer with 0.3% Triton X, and 4% BSA for 24h at room temperature. Secondary antibodies were anti-mouse AlexaFluor488 and anti-rabbit AlexaFluor594 at 1: 1000.